DNA

Part:BBa_K784023:Design

Designed by: Ilya Vainberg Slutskin   Group: iGEM12_Technion   (2012-09-17)


MCS in pSB1C3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 22
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was limited to in length to the single stranded DNA cost. The sites included were the one's that were assumed useful for our team's iGEM project. The PacI site was required for cloning the Lambda phage fragments, since that restriction site was absent in phage genome.

The BglII site overlaps with the XbaI site from the prefix in order to maximize the number of restriction sites in a short sequence of DNA.


Source

Hybridized from two single stranded DNA sequences and cloned into pSB1C3 using the XbaI site and the BioBrick suffix.

References